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Description:Preclinical studyThrough years of efforts, sinobio has achieved the following achievements with independent intellectual property rights in preclinical research:1. In the field of early diagnosis of xenograft rejection, we explored the evaluation and diagnosis technology from molecular biology, cell biology, physiology, histology and imaging;2. Completed the preclinical research on the treatment of diabetic monkeys and methods of islet transplantation. The team led by Professor Wang Wei is the first one in China to complete the research on the treatment of diabetic rhesus monkeys by islet transplantation, which is based on the preliminary work necessary for the clinical application of xenogeneic islet transplantation.3. A inbred line suitable for xenotransplantation of islets of Langerhans was established.4. In the field of xenotransplantation, the preclinical research level (anti rejection program for inducing immune tolerance, cell protection program for non immune damage and image monitoring of immune rejection) of Sinochem is on the same line with foreign counterparts, and the International Xenotransplantation community highly recognizes our team's contribution.5. Established the in vitro culture and expansion system of porcine islet cells with independent intellectual property rights.6. The basic and preclinical study of porcine neural cell transplantation for Parkinson's disease was completed.7. Microencapsulated porcine neurotrophic cells for Parkinson's disease have been developed with independent intellectual property rights.Sinobio has now mastered and innovated various core technologies in the process of islet transplantation for diabetes from donor pig cultivation to islet cell separation and purification, clinical transplantation and anti rejection treatment, some of which have been patented by the state. (please refer to the company introduction column for the attachment of patent certificate)clinical research1. To establish a new comprehensive treatment scheme of anti rejection by xenogeneic cell and tissue transplantation.2. To establish the best interventional puncture point and transplantation site for islet transplantation.3. After the islet cells for transplantation were cultured in our special culture system in vitro, the rejection of islet cells was reduced to a level acceptable to patients, so as to minimize the dosage of anti rejection drugs.4. With the approval of the Ministry of health, the research and development team completed the pre clinical study on the treatment of type 1 diabetes mellitus with porcine islet transplantation, and achieved significant therapeutic effect. International experts had a special discussion on this project, and believed that the research process was convincing. The conclusion is that the transplantation of pig pancreas island is a safe and effective treatment for diabetes, and the project has been at a high level.5. At present, we are entering the countdown phase of standardized xenotransplantation clinical trials.Isolation and purification of islets of LangerhansWe have a cell preparation center and corresponding supporting equipment that meet the clinical requirements and cGMP standards, and use professional cell separation equipment to automatically separate and purify islet cells. Its basic principle is: using the characteristics of different cells with different densities, using the advanced gradient density separation method, the cells can be separated accurately and quickly automatically. At the same time, under the special program, the specific cells with different density have been adsorbed into different collecting tubes, and related treatment has been carried out, so as to achieve the purpose of islet cells and other tissue cells separation.In the process of preparation of porcine islet cells, we use our unique anti rejection technology and islet protection technology to induce immune tolerance, which will reduce the clinical immune rejection to the extent that patients can accept, minimize the amount of anti rejection drugs, and at the same time, the transplanted islet cells are also protected to the maximum extent.After the isolated and purified islet cells are cultured in our special culture system in vitro, these islet cells have four characteristics: ① the survival ability of the transplanted islet cells in human body is enhanced (high activity); ② the insulin secretion function of the transplanted islet cells is enhanced (high efficiency); ③ the immune rejection of human body against the transplanted islet cells is reduced and the biological safety is high (low (4) the number and purity of islet cells were high.Donor pig breedingChina has rich advantages in pig resources. Our preliminary work is mainly to cultivate pig species suitable for xenotransplantation of islets of Langerhans. Through screening in the first five years, we found that there are some special pig species in some closed areas with special topography and special cli